APPLICATION OF AN IMPROVED GLUCURONIDASE ASSAY METHOD TO THE STUDY OF HUMAN BLOOD β-GLUCURONIDASE
نویسندگان
چکیده
منابع مشابه
Application of an improved glucuronidase assay method to the study of human blood beta-glucuronidase.
Previous conditions in which phenolphthalein glucuronide was employed as substrate (1) have proved unsatisfactory for the determination of glucuronidase in plasma, serum, and laked blood cells. Thus, low glucuronidase activities such as frequently occur in plasma could not be accurately measured or even approximated, owing to a combination of circumstances; i.e., too short an incubation period ...
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A continuous spectrophotometric assay has been developed for detecting β-glucuronidase activity. In the assay, Para-nitrophenyl β-D-glucuronide is cleaved to yield a chromophoric product. With the commercial E. coli enzyme, it is demonstrated that the reactions can be continuously monitored by the increase of absorbance at 405 nm. The method is highly sensitive and able to detect less than 1.4 ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1948
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)57416-0